Supplier sign in
Home
STAY INFORMED
Subscribe to our monthly newsletter.
Your email address will never be disclosed to any third party.
Read our privacy notice.

     

ARTICLE
Share this article
Tips for culturing mesenchymal stromal cells
Download article as pdf
If you are on our mailing list, the pdf will be opened directly, otherwise you will be sent a download link.
You also get a sample copy of our newsletter.

Tips for culturing mesenchymal stromal cells

Ideally, cultured cells have the same characteristics as cells that develop in the patientís body. But in practice, there are still many obstacles to be overcome in the development of cellular therapies. To begin with, cultured cells need not only to possess the right weapons to attack a tumor, but they must also move in the right direction. That doesnít always happen, noticed Carlijn Voermans, head of the Cellular Therapy Department at Sanquin, during her research into mesenchymal stromal cell-based the- rapy. Mesenchymal stromal cells are very young cells that mature in the body to become part of cartilage, bone, adipose tissue and other supportive tissues.

Company information

LIFE SCIENCES Tips for culturing mesenchymal stromal cells As the demand for red blood cells declines, the need for white blood cells continues to grow. White blood cells are used for the development and production of cellular therapies, such as T-cell cancer therapy. Sanquin, the Dutch national blood supply organization, is right in step with this trend. Text: Els van den Brink |Translation: Wordís Worth | Photography: FOODnote Ideally, cultured cells have the same characteristics as cells that develop in the patientís body. But in practice, there are still many obstacles to be overcome in the development of cellular therapies. To begin with, cultured cells need not only to possess the right weapons to attack a tumor, but they must also move in the right direction. That doesnít always happen, noticed Carlijn Voermans, head of the Cellular Therapy Department at Sanquin, during her research into mesenchymal stromal cell-based the- rapy. Mesenchymal stromal cells are very young cells that mature in the body to become part of cartilage, bone, adipose tissue and Carlijn Voermans, head of the Cellular Therapy Department at Sanquin other supportive tissues. They play an important role in regulating blood stem cells and bone marrow cells. Voermans explains: ďNormally, injected hematopoietic stem cells automatically remigrate to the bone marrow, but these cultured mesenchymal stromal cells turned out not to do this. When we studied this, we saw that the cultured cells rotate rather than migrate and that the cell nucleus had a weird shape, with a somewhat wrinkled mem- brane.Ē Voermans and her colleagues need to do more research to figure out how to solve this. One possible solution might be to only culture particular subsets of the mesenchymal cells. Microscopic images of the nucleus of regular mesodermal cells (A) and the (wrinkled) nucleus of cultured mesenchymal stromal cells (B). The green color is caused by lamin A/C proteins, architectural proteins that line the inside of the nuclear membrane and determine the shape and size of the nucleus. The blue color is caused by Dapi, a substance that stains nuclear chromatin. AB October 2019 | LABinsights 1
MAXUS MEDIA
LABinsights.net LABinsights.de LABinsights.nl
SIGN UP FOR OUR NEWSLETTER
Newsletter archive
Service and contact
ContactDisclaimerPrivacyAdvertising